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Cell lines and culture All cells were cultured in DME/F12 (50:50) mixed medium supplemented with 10% fetal calf serum, 2 mM glutamine, 1 mM pyruvate, 100 U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C in a humidified incubator under 5% CO 2