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The GLP-1 peptide category in contemporary research therefore refers primarily to DPP-4-resistant analogues engineered with fatty-acid acylation, amino-acid substitutions at position 2 (Aib in semaglutide, tirzepatide and retatrutide), and other structural modifications that extend half-life to 12 hours (liraglutide), ~7 days (semaglutide, tirzepatide, retatrutide) and enable once-weekly dosing protocols in in vivo research models

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