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654 Delivering CRISPR/Cas9 and TYMP cDNA via lipid nanoparticles, polymeric nanoparticles, or AAV2/8 viral vector can efficiently integrate TYMP into the TYMP and Alb loci of hepatocytes in the MNGIE mouse model

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For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT