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At the end of the analysis period, an intravenous bolus injection of the green-fluorescent bile salt analogue cholyl-lysyl-fluorescein (CLF) was performed as a positive control as it is known to be rapidly taken up into hepatocytes and excreted into bile canaliculi (Vartak et al

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Findings thus far have been translated into recommendations in clinical guidelines by the American College of Cardiology, American Heart Association, European Society of Cardiology, and American Diabetes Association

Clinical manifestations of severe deficiency, such as rickets and osteomalacia, are now extremely rare